ΔΔCq qPCR Analyzer

Upload a CSV / XLSX / RDML file from any qPCR instrument, or paste a table. The analyzer detects the format, computes 2^(-ΔΔCq) or Pfaffl, runs the appropriate statistics on ΔCq values, scores reference-gene stability (geNorm / NormFinder / BestKeeper), and lets you download a multi-sheet XLSX, a publication-ready PDF report, and SVG figures.

Drop a CSV / XLSX / TXT / RDML file here, or

Supports Bio-Rad CFX, Thermo QuantStudio / 7500, Roche LightCycler, Qiagen Rotor-Gene, Agilent AriaMx, Analytik Jena qTOWER, Azure Cielo, Mic, plus RDML 1.1–1.4 and generic tidy/wide CSV.

Header autodetect

Recognises Cq / Ct / CT / Cp / Crossing Point / TOP, Sample / Sample Name / Specimen, Target / Gene / Detector / Assay, plus group/replicate/well/dye synonyms.

Strips header-block metadata, pivots wide-format tables, filters standards.

Analysis runs entirely in your browser — Cq data never leaves your device. Statistics follow MIQE 2.0 guidance: tests are performed on ΔCq values (approximately normal); fold changes are reported on the linear scale for display only.